ROS1 Dynamic Range Analyte Control
[HCL035]: ROS1 Dynamic Range Analyte Control (2 unstained slides)
[HCL036]: ROS1 Dynamic Range Analyte Control (5 unstained slides)
[HCL037]: ROS1 Dynamic Range Analyte Control (1 cell microarray block)
IHC using Roche SP384 on the Ventana Benchmark Ultra
ISH using Cytocell ROS1 Plus breakapart
The low expressing glioblastoma cell line contains the FIG-ROS1 fusion, aslso known as GOPC-ROS1. This is an interstitial deletion where the 5' end is lost rather than translocated as seen with SCL34A2-ROS1 where the 5' end resides elsewhere on another chromosome.
It is important to note that the FIG-ROS1 is not identified by all FISH assays. Agilent ROS1 FISH gives a false negative demonstrating overlapping signals. FIG-ROS1 is an interstitial deletion that gives rise to a fusion protein hence it is detected by IHC optimised appropriately.